Credentials

Jose Omar Bustamante, Ph.D.
Director, The Nuclear Physiology Lab
The Millenium Institute of Nanosciences
The National Network of Nanosciences & Nanotechnology

You are visitor number since November 27th of 2001 (day of creation of this page).

Reason for Coming To Brazil – Contract Offer By FMRP-USP

Contract Offer By FMRP-USP

Letter of Recommendation by Yale University to FMRP-USP

Letter of Recommendation by Johns Hopkins University to FMRP-USP

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Curriculum Vitae

Currículo Lattes from CNPq Database (Hypertext HTML format) (Portuguese-Very Complex)

Curriculum Vitae (Adobe's Acrobat PDF Format) (English-8.5x11" Paper)

Curriculum Vitae (Adobe's Acrobat PDF Format) (English-A4 Paper)

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Fellowships, Scholarships & Grants-In-Aid

Note that in all grants-in-aids, Bustamante is the Principal Investigator.
Only the last two had a Co-Principal Investigator.

01.04.83-31.03.84
Digitizer: CAN$11,898
Dalhousie Medical Research Foundation

01.07.83-30.06.85
Calcium Antagonism on Excitation: CAN$62,000
Nova Scotia Heart Foundation (NSHF)
Note: Discontinued due to policy against support simultaneous to that of The New Brunswick Heart Foundation (NBHF). Vide infra.

01.04.84-31.03.87
Single Na-Channels in Adult Cardiomyocytes: CAN$64,096
Natural Sciences and Engineering Research Council(NSERC)
Note: Discontinued due to policy against support simultaneous to that of The Medical Research Council of Canada (MRC-Canada). Vide infra.

01.04.87-31.03.88
Storage Oscilloscope: CAN$12,537
NSERC-Canada

01.07.84-30.06.91
Subproject: Computer Analysis of Human Heart Sodium Currents: CAN$300,000
Project: Electrocardiological Determinants of Cardiovascular Disease: CAN$1,897,725
Medical Research Council of Canada (MRC-Canada)
Note: Discontinued due to change of institution.

01.07.85-30.06.92
Calcium Antagonism on Sodium Channels: CAN$601,878
New Brunswick Heart Foundation (NBHF)
Note: Discontinued due to change of institution.

31.10.91-01.11.92
Nuclear Ion Channels in Cardiac Myocytes: CAN$70,000
University of Ottawa Heart Institute
Note: Discontinued due to change of institution.

01.07.93-31.06.95
Nuclear Ion Channels in Cardiac Myocytes: US$44,000
American Heart Association
Note: Discontinued due to change of institution.

01.10.95-30.11.99
Patch-Clamp Analysis of TATA-Binding Protein Interactions With Nuclear Pores: CAN$136,812
MRC-Canada
Note: Discontinued due to move to Brazil.

01.03.99-01.03.00
-80 oC Freezer + Destilador: R$20,000
Universidade Tiradentes

01.03.00-01.03.02
Biotecnologia da Transfeção: $50,000
Universidade Tiradentes (Aprovada mas, no 27.06.01 ainda por implantar)
Click here to see letter of approval.

17.10.01-16.09.03
Nano Channels – Nuclear Physiol Lab – Millenium Inst Nanosciences
MCT-CNPq (Requested R$250,000 – Release of Funds Pending)
Click here to see approval announcement.
Click here to see proposal.

11.12.01-10.12.03
Nano Channels – Nuclear Physiol Lab – National Nanosciences Network
MCT-CNPq (Requested R$50,000 – Release of Funds Pending)
Click here to see approval announcement.
Click here to see proposal.

12.12.01-11.12.03
Harmful Algae – Nuclear Physiol Lab – Sea & Mangrove Recovery Network
MCT-FINEP (Requested R$150,000 – Release of Funds Pending)
Click here to see approval announcement.
Click here to see proposal.

Fellowships & Scholarships From The Canadian Heart & Stroke Foundation

Grants-In-Aid From The New Brunswick Heart & Stroke Foundation

Letter From The New Brunswick Heart & Stroke Foundation

Letter From The Medical Research Council of Canada (MRC-Canada)-All Grants

Letter From The Medical Research Council of Canada (MRC-Canada)-TBP Grant

Letters From The Natural Sciences and Engineering Research Council of Canada (NSERC)

Grant From The American Heart Association

Honor From The American Heart Association

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Some Documents Related To Academic Posts

Dalhousie University Faculty of Medicine

University of Ottawa Faculty of Medicine

University Maryland at Baltimore - School of Medicine

Yale University School of Medicine

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Research Equipment

Bustamante’s Equipment (Adobe's Acrobat PDF File)

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News On Bustamante’s Research

Major Breakthrough

Research at Heart of Matter

Research at Heart of New Treatments

Canadian Fellowship

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Samples of Publications

List of Publications Indexed by PubMed

Samples of Publications Indexed & Not Indexed by PubMed

1978-First Publication (with Ph.D. Thesis Data)

Dorticós FR, Bustamante JO, Alvarez J & Morlans J (1978)
Effects of atrial premature stimulation on sinus node function in isolated rabbit atria
Cor et Vasa 20: 417-428

1979-Second Publication

Delgado E, Alvarez J, Morlans J, Dorticós FR & Bustamante JO (1979)
Rate-dependent action potential changes in rat atrium
J Physiol (Paris) 75: 729-732

1981-First Method for The Isolation of Normal Myocytes From The Adult Heart

Bustamante JO, Watanabe T & McDonald TF (1981)
Single cells from adult mammalian heart: isolation procedure and preliminary electrophysiological studies
Can J Physiol Pharmacol 59: 907-910

1982-First Use of Non-Specific Proteases for Isolation of Normal Cardiac Myocytes

Bustamante JO, Watanabe T & McDonald TF (1982)
Non-specific proteases: a new approach to the isolation of adult cardiocytes
Can J Physiol Pharmacol 60: 997-1002

1982-First Method for Isolation of Normal Myocytes From Adult Human Heart

Bustamante JO, Watanabe T, Murphy DA & McDonald TF (1982)
Isolation of single atrial and ventricular cells from the human heart
Can Med Assoc J 126: 791-793

1983-First Recordings of Human Heart Sodium Channel Currents

Bustamante JO & McDonald TF (1983)
Sodium currents in segments of human heart cells
Science 220: 320-321

1983-First Study of Myocyte Electrophysiological Heterogeneity

Watanabe T, Delbridge LM, Bustamante JO & McDonald TF (1983)
Heterogeneity of the action potential in isolated rat ventricular myocytes and tissue
Circ Res 53: 280-290

1983-First Demonstration That Calcium Antagonists Block Cardiac Sodium Channels

Bustamante JO (1983)
Rate- and voltage-dependent block of cardiac and neuroblastoma sodium currents by D600
Can Fed Biol Soc 26: PA114

Bustamante JO (1985)
Block of sodium currents by the calcium antagonist D600 in human heart cell segments
Pflügers Arch - Eur J Physiol 403: 225-227

1988-First Data Acquisition System For Fast Electrophysiological Signals With Nuclear Physics Standard (CAMAC)

Bustamante JO (1988)
A system for the automated data-acquisition of fast transient signals in excitable membranes
Int J Biomed Comput 22: 273-283

1988-First Cryopreservation Method of Human Heart Cells

Bustamante JO & Jachimowicz D (1988)
Cryopreservation of human heart cells
Cryobiology 25: 394-408

1989-First Demonstration That Atrial Natriuretic Factor Blocks Cardiac Sodium Channels

Bustamante JO (1989)
Depression of cardiac sodium channel conductance by atrial natriuretic peptide
In: Diuretics III: Chemistry, Pharmacology and Clinical Applications
Proceedings of the Third International Conference on Diuretics
April 2-7, Mexico City, Mexico, 1989, pp. 589-5974
Elsevier, New York. Eds. Puschett JB & Greenberg A

1989-First Application of Scanning Tunneling Microscope (STM) to Ion Channel Imaging

1991-Simple but Efficient Inverted Microscope - Patented by Carl Zeiss

Bustamante JO (1991)
An inexpensive inverted microscope for patch-clamp and other electrophysiological studies at the cellular level
Pflügers Arch - Eur J Physiol 418: 608-610

1991-First Demonstration of Stretch-Activated Channels in Adult Cardiac Myocytes

Bustamante JO, de Bold ML, Boer PH & de Bold AJ (1991)
Presence of stretch-activated channels in freshly isolated adult rat heart cells
J Cell Biochem 15C: H201

1991-First Attempt To Correlate Hypertrophy and Stretch-Activated Channels

Bustamante JO, Ruknudin A & Sachs F (1991)
Stretch-activated channels in heart cells: relevance to cardiac hypertrophy
J Cardiovasc Pharmacol 17(S2): 5110-5113

1992-First Demonstration of Ion Channels In Cardiac Myocyte Nuclei

Bustamante JO (1992)
Nuclear ion channels in cardiac myocytes
Pflügers Arch - Eur J Physiol 421: 473-485

1993-First Publication On The Ion Selectivity of Ion Channels Of Cardiac Myocyte Nucleus

Bustamante JO (1993)
Restricted ion flow at the nuclear envelope of cardiac myocytes
Biophys J 64: 1735-1749

1994-First Description of The Complex Kinetics of Nuclear Ion Channels In Cardiac Myocytes

Bustamante JO (1994)
Open states of nuclear envelope ion channels in cardiac myocytes
J Membr Biol 138: 77-89

1994-First Review On Nuclear Electrophysiology

Bustamante JO (1994)
Nuclear electrophysiology
J Membr Biol 138: 105-112

1994-First Review On The Nuclear Pore Ion Channel Behavior

Bustamante JO, Liepins A & Hanover HA. (1994)
Nuclear pore complex ion channels
Mol Membr Biol 11: 141-150

1994-Review On Apoptosis & Ion Channels

Liepins A & Bustamante JO (1994)
Cell injury and apoptosis
Scanning Microscopy 8: 631-643

1995-First Demonstration of Nuclear Pore Ion Channel Behavior

Bustamante JO, Prendergast RA, Hanover JA, Oberleithner H & Liepins A (1995)
Macromolecular translocation reduces ion flow along nuclear pores:
relevance to measuring transcriptional control mechanisms
J Gen Physiol 105: 25-26a

1995-First Publication Demonstrating Nuclear Pore Ion Channel Behavior.

Bustamante JO, Hanover JA & Liepins A (1995)
The ion channel behavior of the nuclear pore complex
J Membr Biol 146: 239-251

1995-First Detection of Patch-Clamp Detection of Transcription Factor Translocation

Bustamante JO, Oberleithner H, Hanover JA & Liepins A (1995)
Patch-clamp detection of transcription factor translocation along the nuclear pore complex channel
J Membr Biol 146: 253-261

1995-First Publication Combining Atomic Force Microscopy With Patch-Clamp

Bustamante JO, Liepins A, Prendergast RA, Hanover JA & Oberleithner H (1995)
Patch-clamp and atomic force microsocpy demonstrate TATA-binding protein (TBP) interactions with the nuclear pore complex
J Membr Biol 146: 263-272

1996-First Publication of TATA-Binding Protein Imaging With Atomic Force Microscopy

Oberleithner H, Schneider S & Bustamante JO (1996)
Atomic force microscopy visualizes ATP-dependent dissociation of multimeric TATA-binding protein before translocation into the cell nucleus
Pflügers Arch - Eur J Physiol 432: 839-844

1997-Invited Conference At The XXXIII International Congress of Physiological Sciences

1997-Invited Conference At The First European Conference on Nuclear Calcium

1998-Invited Conference At First Workshop of the European Molecular Biology Organization (EMBO)

1998-First Brazilian Publication

Bustamante JO & Varanda WA (1998)
Patch-clamp detection of macromolecular translocation along nuclear pores
Braz J Med Biol Res 31: 333-354

2000-First Demonstration of Role of Ca and ATP on Nuclear Pore Gating

Bustamante JO, Michelette ER, Geibel JP, Dean DA, Hanover JA & McDonnell TJ (2000)
Calcium, ATP and nuclear pore channel gating
Pflügers Arch - Eur J Physiol 439: 433-444

2000-First Use Of Dendrimers To Measure Nuclear Pore Diameter

Bustamante JO, Michelette ER, Geibel JP, Hanover JA, McDonnell TJ & Dean DA (2000)
Dendrimer-assisted patch-clamp sizing of nuclear pores.
Pflügers Arch - Eur J Physiol 439: 829-837

2001-First Review In Physiological Reviews On Nuclear Pores

See cover showing atomic force microscopy of nuclear pores. Mazzanti M, Bustamante JO & Oberleithner H (2001)
Electrical dimension of the nuclear envelope
Physiological Reviews 81: 1-19

2001-First Book Chapter On Nuclear Transport Methods (Including Nuclear Physiology)

Unofficial Site (better images, better housekeeping)
Official Publisher's Site (register and go to Cell Metabolism)

Bustamante JO (2001)
Nuclear Transport Methods
In:Nuclear Import and Export In Plants and Animals.
Eds. V.H. Citovsky & T. Tzvira.
Landes Bioscience/Eureka.Com (Published in Hardcopy and Internet)

2002-First Demonstration of Living Nuclear Pore Ion Channel Behavior

Bustamante JO (2002)
Nuclear pore ion channel activity in live syncytial nuclei
Pflügers Arch - Eur J Physiol - DOI 10.1007/s00424-002-0813-1 Unofficial Site (HTML Galley Proof)
Unofficial Site (PDF Galley Proof)
Original Figures

2002- Biophysical Society´s Chapter on Nuclear Electrophysiology

Bustamante JO (2002)
Recent Advances in Nuclear Electrophysiology
Biophysics Textbook On-Line
Official Download Site of the Biophysical Society
Unofficial Site

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